Quantification of protein expression in dark patches
Quantification of Cy5 protein expression in dark patches.
Download README Download Script Download DataSet Download DataSetDetailed explanation
Quantify channel2 expression inside dark patches found in channel1. 1. Open macro file in Fiji and click Run 2. Input must be a folder with images (or with folders with images inside) that are either .png or .tif files 3. Choose input folder and output folder (main folder containing results) after running the macro 4. Output is a folder with the same name as input image with the following content: zip file with ROIs of dark patches; Csv file with the measurements of each ROI in channel2; and the input image in the composite format. Csv files can be used in Excel for posterior data analysis. Any editable parameters require changing the code itself. Hopefully, each parameter is easy to find by reading the comments existent throughout the macro.
Authors
José Serrado Marques - IGC - Instituto Gulbenkian de Ciência
keywords
ImageJ,Quantification,Fiji,macro
Requirements
Requires Fiji