Name:
intramacrophagic parasites counter

Version:
v00 (IN Cell Developer), v01 (CellProfiler)

Type:


Keywords:
segmentation,nuclei,cells,particles,intracellular,cellprofiler,incell

Requirements:
CellProfiler 3.0 or higher
IN Cell Developer Toolbox 1.9 or higher

Brief Description:
Automated image analysis protocol to count intracellular parasites. Parasites and Nuclei are segmented from the same DAPI channel.

Detailed Explanation:
Functionality: segments nuclei, cells and parasites. Counts nr parasites per cell. To start load .cppipe file on CellProfiler pipeline window or .xeap file on Developer Toolbox. Input: fluorescent images (Nuclei-DAPI, Parasites-DAPI, Cells-Cy5). Main steps: segment nuclei, segment cells, segment parasites, mask parasites to cells, quantification. Outputs: Excel files with quantification + image segmentation outlines + image with nr parasites per cell. editable parameters: adjust for cell and parasites sizes; check segmentation thresholds

DataSet Description:
Bone marrow-derived macrophages (BMDM) infected with L.infantum or L.amazonensis. Macrophages and parasites are labelled with DAPI. Cells are counterstained with HCS CellMask Deep Red stain. Dataset kindly provided by Helena Castro, i3S, Porto.

External Location:
https://github.com/andrefilipemaia/intramacrophagic_parasites_counter

Link To Publication:
<p>https://doi.org/10.1371/journal.pone.0201747</p>


Authors:
André Maia - IBMC - Instituto de Biologia Molecular e Celular,i3S - Instituto de Investigação e Inovação em Saúde