Name:
ToxoGauge

Version:
0.1

Type:
Script/Macro

Keywords:
Toxoplasma,fluorecence quantification

Requirements:
requires ImageJ/FIJI

Brief Description:
Measures semi-automatically size and fluorescence in Toxoplasma gondii parasites in cell cultures 

Detailed Explanation:
Measures semi-automatically size and fluorescence in Toxoplasma gondii parasites in cell cultures 
requires multichannel images, user employs magic wand to identify parasite (PV) and then macro measures size and fluorescence intensity inside and in a cortical rim of the PV. 

DataSet Description:
Images were acquired on a Zeiss Observer, equipped with a 63x Gly PhC 1.3 objective, an Hamamatsu FlashLT camera and a Colibri illuminator and appropriate fluorescence filtersts. 

External Location:


Link To Publication:
<p>https://link.springer.com/protocol/10.1007/978-1-4939-9857-9_20</p>


Authors:
Gabriel G Martins - Instituto Gulbenkian de Ciencia