Name:
DIC_Illumination_Uniformization

Version:
1.0

Type:


Keywords:
Fiji,macro,DIC

Requirements:
Fiji. RGB images

Brief Description:
Macro to uniformize brightness for all DIC images using Retrospective (a posteriori) correction. Useful to have images ready for publication.

Detailed Explanation:
1. Functionality:
Macro uniformize brightness for all DIC images using Retrospective (a posteriori) correction. Transform images into grayscale and puts a scale bar with the size defined by the user. Saves processed image in a new folder, with a name given by the user. Keeps folder structure

2. How to execute:
Open script file in Fiji and click "Run". Follow macro's steps

3. Input
- top folder (directory) with images and/or folders. Those folders must have images inside that are .png or .tif

4. Main steps:
User inputs gray value to uniformize the illumination, output folder name, scale bar size and, if needed, pixel size.

5. Output:
Images with the same illumination value, with a scale bar inserted and within the same folder structure.

6. Editable Parameters
gray value, name of output folder, scale bar size and pixel size.

DataSet Description:
DIC images of Drosophila male gonads. Taken using a Nikon ECLIPSE E400, with the Objective NIKON Japan 40X /0.65 Ph2 DL WD 0.57

Images were kindly provided by Joana Almeida and Paulo Navarro-Costa.

External Location:
https://github.com/zeserrado-marques/Basic-stuff/tree/master/ImageJ%20macro%20code/dic_macro

Link To Publication:
<p>N/A</p>


Authors:
José Serrado Marques - IGC - Instituto Gulbenkian de Ciência